Identification of hCMV antigens in pediatric brain tumors

The University of Chicago Biorepository has only 5-10 ependymoma specimens available for analysis. Based on previous literature analyzing the presence of hCMV antigens in glioblastoma [5, 8] and the possibility of only 33-40% of specimens expressing CMV antigens [7, 10], we estimate 40 specimens are needed to ensure validity. We are requesting 30 from the CBTN. We will also determine hCMV antigen expression in human-derived ependymoma cell lines.