Oncogene-Induced Senescence in RTK-fused Infant High Grade Gliomas

The goals of the project are to compare expression of senescence biomarkers in glioma tissue samples. Senescence refers refers to a cell that is in a stage where they do not divide. Researchers suggest that senescence contributes to the different behaviors and outcomes of infant and pediatric/adult glioma. The mechanism responsible for this difference in senescence induction between infant, pediatric, and adult glioma cells will be further investigated. Using frozen tissue, researchers will compare expression of senescence biomarkers in glioma tissue samples and correlate prevalence of senescent markers with glioma aggressiveness. Using the cell lines, researchers will analyze the effects that genetic manipulations have on senescence and tumor growth in primary human gliocytes and cell lines derived from HGG tissues. The Children’s Brain Tumor Network will make this work possible through the provision of 12 flash frozen tissue samples and 2 suspended cell pellets.