Comparison of Clinical Targeted Next Generation Sequencing (NGS) in FFPE with Whole Genome Sequencing (WGS) of Snap Frozen Pediatric Brain Tumors

DNA sequencing is a process by which scientists identify the base chemicals that make up DNA. The order of these base chemicals act like a computer’s code and serve as instructions for how cells should function. Sequencing the DNA found in tumor samples is important for better understanding cancers and creating treatments. Whole Genome Sequencing (WGS) does this for the entire set of genes and each base present in a tumor cell. Next Generation Targeted Sequencing (NGS) processes target areas in the genome that are known to be actionable and drive tumor mechanisms. Researchers will compare WGS sequencing data and targeted sequencing data to validate the targeting sequencing and potentially develop more precise diagnostics for pediatric brain cancers.