Disclosing chromatin accessibility in brain tumor cells after treatment by cracking a ‘nucleosomal code’

The collection of cell lines at the CBTTC will be invaluable to globally investigate the epigenetic alterations of chromatin in response to epigenetic drugs. We are particularly interested to include in our study the pediatric cell lines with wt histone H3 as well as with the common mutations such as K27M or G34R, to help us to understand the role of histone mutations both in treatment and in chromatin accessibility. In addition, we are very much interested to use the cell lines derived from patients (early passages) to test the response to drugs and corresponding chromatin accessibility profiles, and the CBTTC pediatric cell line collection will be perfect for our aim.