Microglial Mosaicism in BRAFV600E Glioma

Specify why the CBTTC request is important to your project Our proof-of-concept studies in mice, strongly suggest that the presence of somatic mutations in the tumor microenvironment, in our case microglia, contribute to tumor grade. Therefore, we want to test this novel hypothesis in human samples. Since our model is recapitulating a developmental disease, we want to focus on the analysis of samples from pediatric patients, therefore the contribution of a pediatric brain tumor tissue bank is of utmost importance. Obtaining samples from the CBTTC, especially from HGG patients, will allow us to increase the sensitivity of our study. If mutations in microglia are found, we would proceed to the request of clinical information in order to correlate with clinical course. We are currently collaborating with a Pediatric Cancer Tissue Bank in Barcelona, Spain.