CBTN specimen will be used for reduced representation bisulfite sequencing - we will be profiling the transcriptome and epigenome. We will then compare these profiles between boys and girls with pediatric GBM to identify lesions that cause the difference in clinical outcomes of these patients.
This project aims to validate a novel RNA analysis platform. FFPE tissue material will be processed by the HTG EdgeSeq technology pipeline for mRNA and/or miRNA profiling.The HTG EgdeSeq system is an automated chemistry and workflow solution based on the HTG Molecular Diagnostics’ (HTG) ex
Cancers commonly arise as the result of changes affecting the DNA sequence of cells including single nucleotide variations, small insertions and deletions, and structural variations including deletions, duplications, inversions, and translocations. In addition to these, cancer specific splicing a
Childhood craniopharyngiomas are rare tumors usually found near the pituitary gland (a pea-sized organ at the bottom of the brain that controls other glands) and the hypothalamus (a small cone-shaped organ connected to the pituitary gland by nerves).Craniopharyngiomas are usually part soli
Medulloblastomas comprise the vast majority of pediatric embryonal tumors and by definition arise in the posterior fossa, where they constitute approximately 40% of all posterior fossa tumors. Other forms of embryonal tumors each make up 2% or less of all childhood brain tumors.The clinica
High-grade Gliomas (HGG) in children nearly always result in a dismal prognosis. Although novel therapeutic approaches are currently in development, preclinical testing has been limited, due to a lack of pediatric specific HGG preclinical models. These models are needed to help test the effective