Targeting regions of converging synteny in pediatric and canine glioma

CBTTC provided pediatric high-grade glioma data resources and cell lines will be crucial for both of our aims. Specifically, available cell-line and patient WGS and RNA-seq data will be used to identify aneuploidy in conserved synteny regions. Cell-lines will then be subject to low pass WGS to screen for candidate aneuploidy shared with canine glioma derived cell lines. Two of candidate aneuploid cell lines will further be used for CRISPR-Cas9 assay and in-vitro assays to validate effect of gene knock-out.