Central nervous system tumors are leading cause of fatality among children. Current therapeutic approaches focusing primarily on surgical resections and chemotherapy, in even in cases of successful treatment, results in lower standard of life arising from treatment associated cognitive and behavioral disabilities. Hence, there is a pressing need to understand tumor genesis to devise better therapeutic strategies. As part of ERC funded BRAIN–MATCH project we aim generate single cell level neurodevelopmental atlas for human mid and hindbrain; and determine the cell of origin for a repertoire of central nervous system tumors.
What are the goals of this project?
We aim to perform single nucleus RNA-seq (snRNA-seq) using representative regions from human mid and hindbrain over a period of developmental, from embryonic to adult samples. We aim to integrate our neurodevelopmental atlas with transcriptomic profiles of more than 100 central nervous system tumor types to identify tumor type specific cell of origin and developmental window. We will be using in house generated and publically available patient RNA-seq data from various CNS tumor subtypes to make the study comprehensive.
What is the impact of this project?
The results from the proposed study will enhance our understanding of neurodevelopment and identify cell-states/developmental time-points prone to tumor induction.
The Children's Brain Tumor Network contributed to this project by providing access to the Pediatric Brain Tumor Atlas