This collaborative project by CBTN and Parker Institute (PICI) investigators aims to examine the immune and tumor microenvironment in situ in pediatric brain tumor tissues, particularly in response to immunotherapy. Integration of single cell multiplex imaging of tissue microarray targets (TMAs) with existing orthogonal genomic, proteomic, and clinical datasets offer the hope of advancing immunotherapy approaches for pediatric brain tumors.
What are the goals of this project?
The goals of this project are to optimize tumor and immune analysis panels and generate tissue microarrays for analysis, define the expression of protein targets of immunotherapy on tumor cells and other cells in the tumor microenvironment. Define functional phenotypes of tumor-associated myeloid cells and lymphocytes and to integrate tumor-immune protein expression data with clinical and other molecular datasets.
The Children's Brain Tumor Network contributed to this project by providing access to the Pediatric Brain Tumor Atlas.
Crystal L Mackall
Robert Michael Angelo
Pier Federico Gherardini,
Kristina A. Cole, MD, PhD
Kristina Cole, MD, PhD is a pediatric oncologist who has dedicated her career to translational and clinical research, combining her expertise in molecular pathology, cancer genomics, and developmental therapeutics to identify novel treatments for children with cancer. The long-term goal of Dr. Co
Children’s Hospital of Philadelphia