Molecular Analysis of the Cellular Ecosystem of Childhood Ependymoma

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Pablo G. Cámara

CBTN Data Used


About this


Ependymomas constitute nearly one third of the CNS neoplasms among children aged < 3 years. The disease remains a significant therapeutic challenge, with a 5-year overall survival rate < 55 % among this age group and no available chemotherapy of proven efficacy. The breadth of therapies is constrained by the current limited knowledge about the mechanisms of ependymoma progression. This project is part of a large study that utilizes single-nuclei and bulk expression data to characterize the composition and dynamics of the cellular ecosystem of childhood ependymoma.

This project concerns specifically the reconstruction of the immunophenotypic space of childhood ependymoma using available CBTN bulk RNA-seq data. Our preliminary results show that ependymal tumor progression is strongly coupled to alterations in the tumor immunophenotype. We aim to repeat this analysis using CBTN bulk RNA-seq data of 40 childhood ependymomas across all CNS compartments. This will permit us to validate our preliminary results in the CBTN cohort, before generating single-nuclei RNA-seq (snRNA-seq) data of this cohort.

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What are the goals of this project?

The goals are elucidating novel stratification and prognostic schemes of ependymoma patients according to their cellular ecosystems, pinpointing the molecular mechanisms of ependymoma progression, and identifying consequent targets for potential immunotherapeutic intervention

Specimen Data

The Children's Brain Tumor Network contributed to this project by providing access to the Pediatric Brain Tumor Atlas.