Medulloblastoma, the most common form of pediatric brain cancer, is the focus of this project, particularly those cases where the tumor recurs. The knowledge of the pathways and genes that can drive tumors to either progress/recur or be sensitive to treatment can be key for effective cancer treatment. To do this, researchers need to understand the variety within the cancer cells and the cell clones that survive treatment and/or metastasize. Additionally, the knowledge of the interactions between the tumor cells and its microenvironment (the conditions around a cell) is often critical to uncovering the mechanisms of tumor survival. Researchers propose to analyze 17 flash frozen tissue samples and 4 tissue in freezing media samples provided by the Children’s Brain Tumor Network to define cells and genes critical for Medulloblastoma recurrence/progression. Single cell technologies have the potential to substantially propel discovery in the understanding of medulloblastoma cancer biology. Researchers hypothesize that characterization of the cellular and genetic landscape of recurrent/progressive medulloblastomas will reveal important relationships that could lead to the advancement of therapeutic strategies.
What are the goals of this project?
Researchers will analyze medulloblastoma samples in an effort to understand cellular relationships that lead to progression and/or recurrence using techniques that map individual cancer cells.
What is the impact of this project?
Medulloblastoma is the most common form of pediatric brain cancer and this project will propel understanding of its biology leading to advancements in treatment options.
Why is the CBTN request important to this project?
High quality specimens are needed to carry out the work described in this project, and the Children’s Brain Tumor Network will support researchers through provision of such samples.
The Children's Brain Tumor Network contributed 17 flash frozen tissue samples and 4 tissue in freezing media samples for single-cell profiling.
- Ninib Baryawno, Project Manager
- Peter Kharchenko, Bioinformatician
- Cecilia Dyberg, molecular biologist
- Manouk Verhoeven, molecular biologist
- Shenglin Mei, Bioinformatician
Ninib Baryawno, PhD
Single-cell technologies, computational modeling and preclinical testing to study the role of the tumor microenvironment in cancer development, tumor cell dissemination and cancer resistance
Manouk Verhoeven, MSc
Studying immune cells in pediatric cancer.
University of Amsterdam
Shenglin Mei, PhD
Shenglin Mei received his PhD in Bioinformatics from Tongji University under the supervision of Professor Xiaole Shirley Liu. Mei's research was focused on gene transcriptional and epigenetic regulation in cancer through multiple genomic data integration. He is currently working on intratumoral hete
Harvard Medical School
Cecilia Dyberg, MD
Peter Kharchenko, PhD
Peter Kharchenko received a PhD in biophysics at Harvard University, studying gene regulation and metabolic networks under the advisement of George Church. He then completed a four-year postdoctoral fellowship in computational biology and genomics in the laboratory of Peter Park. He is currently stu
Harvard Medical School
Medulloblastomas comprises the vast majority of pediatric embryonal tumors and by definition arise in the posterior fossa, where they constitute approximately 40% of all posterior fossa tumors. Other forms of embryonal tumors each make up 2% or less of all childhood brain tumors.The clinical feature