Delineating Pediatric Glioma Progression Using Single-nuclei Sequencing

Email Principal Investigator
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Trevor Pugh

University of Toronto
Toronto, ON M5T 1P5, Canada

CBTN Specimen


CBTN Samples


Alex's Lemonade Stand Foundation, Single-cell Pediatric Cancer Atlas

About this


Low-grade gliomas are the most prevalent brain cancer among children. While patients with this type of cancer respond well to surgery, the majority experience recurrence after initial removal and require additional rounds of treatment. Deeper study is needed to better steer and prioritize post-removal treatment protocol. For the past year, researchers have been studying the immune microenvironment of over 1100 samples of pediatric cancers including those provided by the Children’s Brain Tumor Network. The overall aim of this proposal is to understand changes in malignant and non-malignant compartments of pediatric low-grade gliomas during the course of the disease in an effort to guide treatment. The goals of this project are to determine changes in tumors during the course of tumor progression and to investigate contribution and evolution of tumour microenvironment in the low-grade glioma. The Children's Brain Tumor Network will be contributing to this project by providing rare, high quality low grade glioma samples. By studying cancer and non-cancerous cell types that populate brain tumours upon recurrence, researchers expect to identify novel therapy targets and to develop combination therapy protocols informed by this work.

Ask The


Ask the scientists

What are the goals of this project?

Researchers seek to understand how low grade gliomas change in their immune tumor microenvironment throughout progression and treatment and how the tumor microenvironment supports the tumor’s growth and progression in an effort to inform more personalized treatment protocol.

What is the impact of this project?

Through a better understanding of the changes undergone by low grade gliomas in the tumor microenvironment over the course of treatment, researchers hope to better optimize the use of single and combined therapies.

Why is the CBTN request important to this project?

The high quality low grade glioma samples available provided by the Children’s Brain Tumor Network are integral to the in depth nature of this study.

Specimen Data

The Children's Brain Tumor Network will be contributing to this project by providing low grade glioma samples.