Childhood brain cancer is a devastating disease that is still poorly understood. Glioma is a type of brain cancer in which a specific type of cells in the brain (called glial cells), become cancerous. In our study, we will examine how the DNA in glioma cells varies between different groups of patients. We will also study how these differences in DNA affect gene expression. This will allow us to gain a greater understanding of how pediatric glioma develops, how it differs between patients, and how childhood glioma differs from adult glioma. In our research, we will utilize RNA sequencing data from pediatric gliomas from the CBTN datasets to examine glioma gene expression.
What are the goals of this project?
In our study, we are searching for previously uncharacterized molecular subtypes of pHGG. Specifically, we will integrate the DNA methylation and transcription landscapes of pHGG to search for distinct molecular subtypes that are associated with differences in prognosis and/or specific clinical covariates. Next, we will stratify pHGG samples by clinical covariates and compare their molecular profiles with those of controls to delineate previously unknown differences that may drive tumorigenesis.
Why the CBTN request is important to this project?
The CBTN data (specifically the RNA sequencing data from pHGG samples) will be integral to the characterization of pHGG transcription landscape in our study.
Jeffrey Greenfield, MD, PhD
Dr. Greenfield’s directs multiple research projects pertaining to pediatric brain tumors, in particular, basic research examining the brain tumor microenvironment, tumor immunology, and precision medicine. This research has been externally funded and widely published and he has received national
Weill Cornell Medicine Pediatric Brain & Spine Center
High-grade glioma/astrocytoma (WHO grade III/IV)
High-grade Gliomas (HGG) in children nearly always result in a dismal prognosis. Although novel therapeutic approaches are currently in development, preclinical testing has been limited, due to a lack of pediatric specific HGG preclinical models. These models are needed to help test the effective
Brainstem glioma- Diffuse intrinsic pontine glioma
A presumptive diagnosis of DIPG based on classic imaging features, in the absence of a histologic diagnosis, has been routinely employed. Increasingly however, histologic confirmation is obtained for both entry into research studies and molecular characterization of the tumor.[