Analysis of Chromatin Pathways as Regulators of High Grade Glioma Gene Expression Patterns

Email Principal Investigator
Ongoing
Data
HGG
DIPG

About this

Project

We plan to identify genes that are up- and down-regulated in brain tumors compared to normal tissue samples and genes that are up- and down-regulated in brain tumors with different mutations. We will compare these patient-derived gene expression profiles to data we have generated using human brain cancer cells grown in the lab that have either been genetically manipulated, or treated with various drugs.

Ask The

Scientists

Ask the scientists

What are the goals of this project?

The goals of this project are to identify genes that are differentially expressed in pediatric brain tumors compared to normal tissue, to identify genes that are differentially expressed in brain tumors with different mutations (eg. tumors with wild type histones compared to H3.3/H3.1 K27M and H3.3 G34R/V mutant tumors), and to compare the above gene signatures to RNAseq data that we have generated in our lab using cultured human brain cancer lines, which have been either genetically manipulated or treated with various small molecule inhibitors to target different chromatin and epigenetic regulators.

What is the impact of this project?

By analyzing and comparing patient and laboratory datasets we hope to identify gene expression patterns characteristic of different subsets of pediatric brain tumors, and to investigate the potential consequences of our genetic manipulation and drug treatment studies, which could provide a theoretical basis for the development of new therapies.

Specimen Data

The Children's Brain Tumor Network contributed to this project by providing access to the Pediatric Brain Tumor Atlas.

Explore the data in these informatics portals

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