Project Hope: High-Grade Glioma-Omics in Pediatric and AYA

Email Principal Investigator
Ongoing
Specimen
HGG
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Adam Resnick

Children’s Hospital of Philadelphia
Philadelphia, PA, USA

CBTN Samples Used

84

CBTN Participants

109

CBTN Samples

Backer

NCI

About this

Project

Our overall objective of the project is the implementation of single-cell sequencing techniques in the context of a multiomics approach that includes bulk WGS, RNAseq, and total/phospho proteomics to investigate pediatric and AYA HGG biology. Analyses will focus on biospecimens collected across multiple surgical events and/or specimens collected from patients defined as extreme short survivors. Analyses will evaluate dynamic changes across time in the bulk expression and genomics as well as the microenvironment, and the immunologic status of tumors . We hypothesize that such integrated multi-omic analysis will lead to etter understanding of these diseases and the development of more effective therapeutics to improve outcomes for patients.

Ask The

Scientists

Ask the scientists

What are the goals of this project?

The goals of this project are to generate single-cell sequencing data of cancer cells, infiltrating immune cells and non-immune microenvironment in pediatric and AYA high-grade gliomas and epigenetic profiling of matched samples by EPIC (850K) arrays or whole genome bisulfite sequencing.

What is the impact of this project?

Our overall hypothesis is that a focus on single-cell techniques, the microenvironment, and the immunologic status of tumors and matched preclinical models will lead to a better understanding of these diseases and the development of more effective therapeutics to improve outcomes for patients.

Why the CBTN request is important to this project?

The Children's Brain Tumor Network provides a unique resource of samples, genomic data and longitudinal clinical data.

Specimen Data

The Children's Brain Tumor Network contributed to this project by providing tissue samples.

Meet The

Team