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Adam Resnick

Children’s Hospital of Philadelphia
Philadelphia, PA, USA


CBTN Specimen


CBTN Participants


CBTN Samples

About this


Pediatric brain tumors are the leading cause of disease related death in children. Major factors contributing to treatment failures for children with brain tumors include: i) the lack of comprehensive molecular description of the disease and an associated dearth of integration of the tumors’ biological signatures at gene, protein and RNA levels; ii) paucity of comprehensive and molecularly-driven preclinical tests for informing precision-targeting disease pathways; and iii) lack of sensitive methods of assessing tumor response to treatment. Furthermore, while surgery remains the mainstay of pediatric brain tumor treatment, many tumors are non-resectable and/or progressive/disseminated tumors lead to significant tumor-related morbidity. In contrast to adults, tumors in central nervous system are often challenging to treat with traditional chemotherapy and radiation resulting in long-term neuro-toxicities in the context of the developing brain.

While pediatric cancers have benefited from large-scale genomics efforts lead by the NIH in the context of TARGET (Therapeutically Applicable Research To Generate Effective Treatments), brain tumors are not represented in these cohorts. The consortia-based efforts of the Children's Brain Tumor Network and the Pacific Pediatric Neuro-Oncology Consortium and associated platforms were complemented with the launch of CAVATICA (, a scalable, cloud-based storage and compute environment providing comprehensive, secure, and integrated access to pediatric cancer genomic data that is further integrated with additional rare-disease-genomic data. These early efforts are now undergoing significant expansion through the Commons Fund’s Kids First Program and CHOP’s role in leading the development of the Kids First Data Resource Center (DRC) along with its partners at the Ontario Institute for Cancer Research, the University of Chicago, Children’s National Health System, the Oregon Health and Science University and Seven Bridges. The Kids First DRC will support accelerated scientific discovery across more than >20,000 WGS from dozens of childhood cancer and structural birth defects cohorts being sequenced over the next 2 years. It is in this context of emerging genomic data and a data commons architecture that we propose to define a discovery cohort for comprehensive proteomic analysis in pediatric brain tumors.

Ask The


Ask the scientists

What are the goals of this project?

We propose to perform comprehensive quantitative and associated post translational analyses on a pilot cohort

What is the impact of this project?

Our own and other’s efforts in characterizing pediatric brain tumors have defined their biologic underpinnings as distinct from adult brain tumors. Compared to adults, pediatric cancers possess significantly fewer driver mutations. Furthermore, in addition to canonical respective loss/gain of tumor suppressors/oncogenes, gene fusions events provide a common driving genetic mechanism. However, such structural variations also impinge on the epigenetic landscape in ways only recently appreciated. Indeed, especially in high-grade tumors, epigenetic dysregulation provides a common and ubiquitous paradigm for pediatric brain tumors likely informed by the developmental context of the central nervous system. Our own recent proteomic analyses of DIPGs, where histone mutations (“onco-histones”) have been identified, have focused on histone posttranslational modification and the potential therapeutic role of HDACs in modulating such epigenetic dysregulation. To date, few proteomic studies have been defined for pediatric cancer.

Why is the CBTN request important to this project?

The CBTN collects longitudinal, deep clinical, and phenotypic data on all prospective patients. More than 900 distinct flash-frozen specimens have been collected since 2011 across all brain tumor histologies with >75 mg of tissue and paired blood (germline) and are undergoing WGS/RNAseq (~200 samples have been completed).

Specimen Data

The Children's Brain Tumor Network contributed to this project by contributing over 200 samples and by providing access to the Pediatric Brain Tumor Atlas.

Explore the data in these informatics portals


Meet The


● Fred Hutch: Amanda Paulovich

● Harvard: Steven Gygi

● NIH: Kinsinger, Christopher (NIH/NCI)

● NIH: Rodriguez, Henry (NIH/NCI)

● NIH/NCI: Boja, Emily (NIH/NCI)

● NIH/NCI: Bocik, William (NIH/NCI)


Scientific Committee

Executive Board

Scientific co-Chair

Principal Investigator

Adam Resnick, PhD

Adam Resnick is the Director of Data Driven Discovery in Biomedicine (D3b) at Children’s Hospital of Philadelphia (CHOP) responsible for leading a multidisciplinary team to build and support a scalable, patient-focused healthcare and educational discovery ecosystem on behalf of all children. He is a


Children’s Hospital of Philadelphia

Philadelphia, PA, USA

Scientific Committee

Executive Board

Executive co-Chair

Principal Investigator

Brian Rood, MD

Brian R. Rood, MD, is an Associate Professor of Pediatrics in the Center for Cancer and Blood Disorders at Children’s National Hospital whose primary clinical focus is pediatric neuro-oncology. Dr. Rood joined the faculty of Children’s National in 2002 after completing a fellowship in Pediatric Hema


Children’s National Hospital

Washington, DC, USA
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Scientific Committee

Executive Board

Principal Investigator

Javad Nazarian, PhD, MSc

I am an investigator at the Center for Genetic Medicine in Children’s National Hospital in Washington, D.C., and an assistant professor in Integrative Systems Biology at the George Washington University. I received my PhD from the George Washington University in Genetics in 2005. My dissertation res


Children’s National Hospital

Washington, DC, USA

Amanda Paulovich, MD, PhD

The Paulovich Laboratory is a multidisciplinary team focused on improving patient care by enabling the reliable quantification of proteins in a CLIA environment. Quantifying proteins is important for matching patients to effective therapies and for the development of new therapies to treat cancer an


Steven Gygi

Research in the Gygi lab centers around developing and applying new technologies in the field of mass spectrometry-based proteomics.  These include the systematic and proteome-wide measurements of many protein properties including their expression levels, modification states, structure, localizatio


Christopher Kinsinger, PhD

Dr. Kinsinger focuses on the expansion and coordination of open data access and programmatic goals involving mass spectrometry, informatics, and biospecimens.  In this role, he works with NCI staff and investigators to optimize proteomics technology, establish policies for sharing data and biospeci


Henry Rodriguez, PhD, MS, MBA

Dr. Rodriguez has led the development of NCI’s clinical proteomic and proteogenomic research programs, which today includes the world’s largest public repository of proteogenomic sequence data and targeted fit-for-purpose assays.  These efforts led to the formation of two Cancer Moonshot initiative

Emily Boja.jpeg

Emily Boja, PhD

Dr. Boja leads the precisionFDA’s biomedical competitions around omics science, as well as outreach activities in building a community of scientists around the analysis of biological datasets to inform regulatory science and advance precision medicine. Prior to joining OHI, she was a Program Direct


William Bocik, PhD

Dr. Bocik assists in coordinating both proteogenomic informatics research strategies and assay development efforts within the CPTAC program.

nci 2.jpg

National Cancer Institute, National Institute of Health

NCI Shady Grove Campus, 9609 Medical Center Drive, Rockville, MD 20850





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